摘要：The determination of small amounts of D -amino acids in mammalian tissues is still a challenging theme in the separation sciences. In this review, various gas-chromatographic and high-performance liquid chromatographic methods are discussed including highly selective and sensitive column-switching procedures. Based on these methods, the distributions of D -aspartic acid, D -serine, D -alanine, D -leucine and D -proline have been clarified in the mouse brain. As the regulation mechanisms of D -amino acid amounts in mammals, we focused on the D -amino-acid oxidase, which catalyzes the degradation of D -amino acids. Using the mutant mouse strain lacking D -amino-acid oxidase activity, the effects of the enzymatic activity on the amounts and distributions of various D -amino acids have been investigated.
关键词：D -amino acid; D -amino-acid oxidase;chiral separation;HPLC;ddY/DAO− mouse
