摘要：Manullang JR, Nugroho RA, Rohmah M, Rudianto, Qorysuchi A2021Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterizationNusantara Bioscience 13: 247-254The plant-based biosynthesis of nanoparticles has gained increasing momentum due to being lower in cost and eco-friendlyThis study aimed to biosynthesize nanoparticles from the bulb ethanolic extract of Eleutherine americana (Aubl.) Merrex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacityThe Ea-AgNPs were synthesized using ethanolic extract of Eamericana bulb along with various concentrations of AgNO3 (0.5-4 mM)The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/ Energy Dispersive X-ray (SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR) techniquesThe results indicated that Eamericana can be used to reduce AgNO3 to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400 nmThe FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important biocompoundsAdditionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 ThetaThe particle size distribution curve of Ea-AgNPs showed a size of 105 nmFurthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keVThe EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and Ag atom (0.06 %)The TEM imaging also showed the characteristics of Ea-AgNPsSome phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, with IC50 values of 45.30 ppm.
