摘要：Six diarylheptanoids (1—6) from the stem bark of Alnus hirsuta were investigated for their inhibitory activity against LPS-induced NF- k B activation and NO and TNF-α production. Among them, compounds 2, 3, and 6 displayed inhibitory activity against NF- k B activation and NO and TNF-α production with IC50 values of 9.2—9.9 μ M , 18.2—19.3 μ M , and 22.3—23.7 μ M , respectively, in RAW264.7 cells. Three active compounds had no significant cytotoxicity in RAW264.7 cells at their effective concentrations. This is the first report of NF- k B-inhibitory activity of these compounds and supports the pharmacological use of A. hirsuta , which has been employed as a herbal medicine for the treatment of inflammatory diseases.
关键词：Alnus hirsuta;diarylheptanoid;NF-kB activation;NO production;TNF-α production
