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  • 标题:Identification of Armillaria nabsnona in Gastrodia Tubers
  • 本地全文:下载
  • 作者:Haruo Sekizaki ; Shiro Kuninaga ; Mizuho Yamamoto
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2008
  • 卷号:31
  • 期号:7
  • 页码:1410-1414
  • DOI:10.1248/bpb.31.1410
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The symbiosis between Armillaria species and an achlorophylous orchid Gastrodia elata B LUME has been reported. The main species described as a symbiont is Armillaria mellea (V AHL : F R .) K UMMER , known widely as a primary root rot pathogen. Samples collected from the rhizomorphs attached to the tuber of G. elata were separated and analyzed. Molecular analysis based on sequencing of the intergenic spacer 1 (IGS-1) and internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) was performed, coupled with restriction fragment length polymorphism (RFLP) of the IGS-1 region. Cultural morphology and features of basidiomata were also used to characterize the isolates. Phylogenetic analysis and morphological data strongly suggested that the fungus present in the tubers of G. elata is Armillaria nabsnona . This is the first report of occurrence of this Armillaria species in association with G. elata .
  • 关键词:Armillaria nabssnona;Gastrodia elata;symbiosis;molecular analysis;intergenic spacer one;internal transcribed spacer
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