摘要：Recently, we showed that a biosurfactatnt 4- O -[(4′,6′-di- O -acethyl-2′,3′-di- O -alkanoyl)-β- D -mannopyranosyl] meso -erythritol A (MEL-A) greatly increased the efficiency of gene transfection mediated by cationic liposomes in vitro . We then studied whether the high transfection efficiency of these liposomes is maintained in vivo for tumor cells in the mouse abdominal cavity. When a complex of the liposomes and plasmid DNA was injected intraperitoneally into C57BL/6J mice bearing B16/BL6 tumors, we found that the biosurfactant significantly increased liposome-mediated gene transfection to the mouse tumor cells. The transfection efficiency of the plasmids into the solid tumors by the cationic liposomes of cholesteryl-3β-carboxyamidoethylene- N -hydroxyethylamine (OH-Chol) with MEL-A increased by about 100-fold compared with that by the cationic liposomes of DC-Chol (commercially available) without MEL-A. The results suggest that nonviral vectors with MEL-A are very useful for gene transfection in vivo .
