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  • 标题:Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-Like Epithelial Cells
  • 本地全文:下载
  • 作者:Mika Ikehata ; Ryoko Yumoto ; Yuki Kato
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2009
  • 卷号:32
  • 期号:10
  • 页码:1765-1769
  • DOI:10.1248/bpb.32.1765
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The mechanism underlying the handling of protein and peptide drugs such as insulin in alveolar epithelial cells is still unclear. We therefore examined fluorescein isothiocyanate-labeled (FITC)-insulin uptake in rat primary cultured alveolar type II epithelial cells and in transdifferentiated type I-like cells. FITC-insulin uptake in these cells was much higher than those of FITC-immunoglobulin (IgG), transferrin, and dextran. FITC-insulin uptake was time- and concentration-dependent, and was almost completely inhibited by metabolic inhibitors in both cells, while bafilomycin A1 inhibited the uptake only in type II cells. Inhibitors of clathrin- and caveolae-mediated endocytosis did not affect FITC-insulin uptake in either type of cell. Dynasore, a dynamin GTPase inhibitor, potently inhibited FITC-insulin uptake in type II cells. These results suggest that the characteristics of insulin uptake in type II and type I cells are different, and dynamin-dependent endocytosis that utilizes neither clathrin nor caveolae is involved in type II cells, while a dynamin-independent pathway is mainly involved in type I cells.
  • 关键词:insulin;alveolar epithelial cell;endocytosis;primary culture;dynamin
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