标题：Molecular Authentication of 21 Korean Artemisia Species (Compositae) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Based on trnL–F Region of Chloroplast DNA
摘要：The present study describes the molecular authentication of 21 Korean Artemisia species using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) technique based on the trn L–F sequences in chloroplast DNA. Five different banding patterns were generated from 21 Artemisia species using Hinf I restriction enzyme. A. apiacea , A. keiskeana and A. sieversiana have specific banding patterns. The remaining 18 species had shared two banding patterns. Phylogenetic analysis based on trn L–F sequence variations showed results similar to PCR-RFLP banding patterns. It suggested that the trn L–F region does not have sufficient variations to identify the 21 Artemisia species. However, the specific banding patterns for A. apiacea , A. keiskeana and A. sieversiana can be utilized as a DNA marker for discriminating them from other Artemisia species. These markers will be also useful for developing A. apiacea , A. keiskeana and A. sieversiana into new medicine and food based on their efficacy.
关键词：Compositae;Artemisia L.;trnL;trnF;polymerase chain reaction-restriction fragment length polymorphism
