标题：Goshuyuto, a Traditional Japanese Medicine, and Aqueous Extracts of Evodiae Fructus Constrict Isolated Rat Aorta via Adrenergic and/or Serotonergic Receptors
摘要：Effects of goshuyuto, a traditional Japanese medicine, on vascular constriction were examined using isolated strips of rat aorta. Goshuyuto (1×10−5 to 1×10−3 g/ml) caused constriction of aorta strips in a dose-dependent manner. The vasoconstrictive effects of goshuyuto were significantly inhibited by pretreatment with prazosin, an adrenergic α1 receptor antagonist. The constrictive effects were partially inhibited by pretreatment with BRL15572, a 5-HT1D antagonist, and ketanserin, a 5-HT2A antagonist. However, the constrictive effects were not inhibited by pretreatment with SB216641, a 5-HT1B antagonist, or propranolol, an adrenergic β receptor antagonist. In addition, aqueous extracts of Evodiae Fructus , one of the constituent medicinal herbs of goshuyuto, caused constriction of aorta strips strongly, but aqueous extracts of Zizyphi Fructus , Ginseng Radix , and Zingiberis Rhizoma , the other constituents of goshuyuto, did not have much effect on the vascular response of rat aorta strips. Also, synephrine, which is one of the ingredients of Evodiae Fructus , constricted the rat aorta. These results suggest that goshuyuto constricts rat aorta strips and that the mechanisms involve the adrenergic and/or serotonergic receptors. Also, it may be suggested that Evodiae Fructus and synephrine play the important role in the vasoconstrictive effects of goshuyuto on rat aorta strips.
