摘要：Through screening for natural ligands against peroxisome proliferator-activated receptor γ (PPARγ) using the PPARγ luciferase reporter assay, 6 hydroxy unsaturated fatty acids were isolated from adlay seed ( Coix lacryma-jobi L. var. ma-yuen S TAPF. ) extracts with acetone and 70% ethanol. The structures of these compounds were determined via spectral analysis as 13-hydroxy-(9 E ,11 E )-octadecadienoic acid (13- E , E -HODE) (1), 9-hydroxy-(10 E ,12 E )-octadecadienoic acid (9- E , E -HODE) (2), 9-hydroxy-(10 E )-octadecenoic acid (3), 10-hydroxy-(8 E )-octadecenoic acid (4), 8-hydroxy-(9 E )-octadecenoic acid (5), 11-hydroxy-(9 Z )-octadecenoic acid (6). 9- E , E -HODE (2) exhibited the most potent PPARγ agonist activity of the isolated hydroxy unsaturated fatty acids. 9- E , E -HODE (2) and 13- E , E -HODE (1) are the respective geometrical isomers of 9-hydroxy-(10 E ,12 Z )-octadecadienoic acid and 13-hydroxy-(9 Z ,11 E )-octadecadienoic acid, both of which are likely to be natural PPARγ agonists produced in various mammalian cells, suggesting that 9- E , E -HODE may also act as PPARγ agonist.
