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  • 标题:Preventive Effects of Salvianolic Acid B on Transforming Growth Factor-β1-Induced Epithelial-to-Mesenchymal Transition of Human Kidney Cells
  • 本地全文:下载
  • 作者:Gang Yao ; Lizhi Xu ; Xiaochun Wu
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2009
  • 卷号:32
  • 期号:5
  • 页码:882-886
  • DOI:10.1248/bpb.32.882
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Epithelial-mesenchymal transition (EMT) is an important mechanism in kidney fibrosis. While Salvianolic acid-B (Sal B) has been well appreciated to show a protective effect of tissue fibrosis, the objective of this study was to investigate the influence of Sal B on the transdifferentiation of renal tubular epithelial cells. Human kidney proximal tubular cell line (HK-2) was used as the proximal tubular cell model and EMT was induced with 5 ng/ml of human tranforming growth factor (TGF)-β1. The effects of the Sal B on cell morphology were observed by phase contrast microscopy, and the possible mechanisms were studied by immunocytochemistry and real-time reverse transcription-polymerase chain reaction. Our results revealed that Sal B could inhibit TGF-β1-induced myofibroblast phenotype and restored the epithelial morphology in a dose-dependent manner. It was partially through modulating α-smooth muscle actin (SMA) increase and E-cadherin reduction. These observations strongly suggest that Sal B is a potent inhibitor of TGF-β1-induced EMT and might be a promising agent for treating tubulointerstitial fibrosis.
  • 关键词:Salvianolic acid-B;epithelialto-mesenchymal transition;kidney fibrosis
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