标题：Anti-clastogenic Effect of Magnolol-Containing Hange-koboku-to, Dai-joki-to, Goshaku-san, and Magnoliae Cortex on Benzo(a)pyrene-Induced Clastogenicity in Mice
摘要：Magnolol was previously shown to inhibit genotoxicity induced by environmental mutagens both in vitro and in vivo . Here, we investigate the effects of the magnolol-containing kampo (traditional) medicines Hange-koboku-to , Dai-joki-to , and Goshaku-san , as well as Magnoliae Cortex , on the clastogenesis induced by benzo( a )pyrene (B( a )P) using the mouse micronucleus test. The mice were first treated with a single intraperitoneal injection of B( a )P, followed by a single oral dose of Hange-koboku-to , Dai-joki-to , Goshaku-san , or Magnoliae Cortex . Peripheral blood specimens were prepared 48 h after B( a )P administration and analyzed using the acridine orange (AO) technique. The anti-clastogenic mechanisms employed by the kampo medicines and Magnoliae Cortex were also investigated by evaluating in vivo CYP1A1 activity using the zoxazolamine paralysis test. Results show that Hange-koboku-to , Dai-joki-to , and Magnoliae Cortex , which contain high levels of magnolol, significantly inhibited the clastogenesis induced by B( a )P and sufficiently inhibited in vivo CYP1A1 activity. In contrast, Goshaku-san , which contains low levels of magnolol, had little inhibitory effect on clastogenicity and in vivo CYP1A1 activity. These findings suggest that magnolol is a major contributor to the inhibition of B( a )P-induced clastogenesis, and that kampo medicines exert significant anti-clastogenic effects.
