摘要：The leaves of three Ligularia species belonging to the family Compositae, Ligularia stenocephala , L. fischeri , and L. fischeri var. spiciformis , were qualitatively and quantitatively analyzed on the caffeoylquinic acids by HPLC and subjected to peroxynitrite-scavenging assay. The IC50 of the MeOH extract of L. stenocephala was 1.62±0.03 μg/ml and the major caffeoylquinic acids of L. stenocephala were 5- O -caffeoylquinic acid, 3,5-di- O -caffeoyl- muco -quinic acid, and 3,5-di- O -caffeoylquinic acid. The compositions of caffeoylquinic acids were different for the three plants. Since percentage of total caffeoylquinic acids of the extract was highest (42.20% of the MeOH extract and 94.52% of the BuOH extract) in L. stenocephala and potent in peroxynitrite-scavenging assay, the extracts of L. stenocephala were chosen to perform in vivo anti-ulcerogenic activity. Treatment of mice with the MeOH- and BuOH extracts decreased the diameter of gastric lesions caused by HCl/ethanol- and indomethacin/bethanechol and decreased the volume of gastric juice, suggesting that caffeoylquinic acids have anti-ulcerogenic activity. These results suggest that the leaves of Ligularia species may help prevent or treat gastric ulcers.
