摘要：Invariant natural killer T ( i NKT) cells regulate multi-immune response through Th1/Th2 cytokine release triggered by the recognition of CD1d-restricted glycosphingolipid antigens. Here we report that acidic glycosphingolipids (AGLs) of mushroom ( Hypsizigus marmoreus and Pleurotus eryngii ) presented by murine CD1d-transfected rat basophilic leukocytes induced interleukin-2 (IL-2) release from i NKT hybridoma cells. AGL-1, one of the AGLs, containing mannose at the non-reducing ends, induced CD1d-dependent IL-2 release. Al-though α-galactosylceramide (α-GalCer) presented by CD11c-positive cells induced both interferon-γ (IFN-γ) and IL-4 release, all of AGLs presented by CD11c-positive cells and AGL-1 presented by B cells induced IL-4 release from i NKT hybridoma cells. A single intravenous injection of AGLs into B6 mice induced only a little elevation of IL-4 in serum but repeated intravenous injection of AGLs induced prolonged retention of IL-4 in serum; therefore, these results suggested that edible mushroom AGLs might contribute to the retention of immunohomeostasis through the minimum induction of i NKT cell activation in vivo .
关键词：alpha-galactosylceramide;glycosphingolipid;edible mushroom;natural killer T cell;mouse
