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  • 标题:Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain
  • 本地全文:下载
  • 作者:Kosuke Nakamura ; Hiroshi Akiyama ; Kiyomi Ohmori
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2011
  • 卷号:34
  • 期号:10
  • 页码:1648-1651
  • DOI:10.1248/bpb.34.1648
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Unauthorized genetically modified (GM) papaya ( Carica papaya L INNAEUS ) was detected in a commercially processed product, which included papaya as a major ingredient, in Japan. We identified the transgenic vector construct generated based on resistance to infection with the papaya ringspot virus (PRSV) YK strain. A specific detection method to qualitatively monitor papaya products for contamination with the GM papaya was developed using the real-time polymerase chain reaction.
  • 关键词:genetically modified organism;papaya;polymerase chain reaction;genomic DNA
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