标题：Modification and Translocation of Rac/Rop Guanosine 5′-Triphosphate-Binding Proteins of Scoparia dulcis in Response to Stimulation with Methyl Jasmonate
摘要：Translocation of two Rac/Rop guanosine 5′-triphosphate-binding proteins from Scoparia dulcis , Sdrac-1 and Sdrac-2, was examined employing transformed belladonna which overproduces these proteins as glutathione- S -transferase-tagged forms. The transferase activities of the fused proteins in microsomal fraction of belladonna markedly increased by the incubation with methyl jasmonate either in Sdrac-1 or Sdrac-2 transformant, while low and constant activities were observed in the untreated control. Recombinant Sdrac-2 protein was found to bind to prenyl chain in the presence of cell extracts prepared from methyl jasmonate-treated S. dulcis , however, Sdrac-1 was palmitoylated by the addition of the cell extracts. These results suggest that both Sdrac-1 and Sdrac-2 translocate to plant membranes by the stimulation with methyl jasmonate, however, targeting of these proteins is triggered by the independent modification mechanisms, palmitoylation for Sdrac-1 and prenylation for Sdrac-2.
关键词：methyl jasmonate;Rac protein;plant signal transduction;intracellular translocation;prenylation;palmitoylation
