摘要：Allergy-preventive activity of flower buds of Lonicera japonica T HUNB . was found in the 35% EtOH extract (LJ) using an in vivo assay, The assay system uses monitoring of a decrease in blood flow (BF) in the tail vein of mice subjected to sensitization with hen-egg white lysozyme (HEL). Bioassay-guided fractionation of the 35% EtOH extract led to isolation of chlorogenic acid ( 1 ) and three known iridoid derivatives, loganin ( 2 ), secoxyloganin ( 3 ) and sweroside ( 4 ), all of which inhibited the BF decrease. This suggested that the flower buds of L. japonica and compounds isolated from them have allergy-preventive properties. The structure–activity relationship of iridoid derivatives, morroniside ( 5 ), geniposide ( 6 ), asperuloside ( 7 ), aucubin ( 8 ) and catalpol ( 9 ), were also tested using the same bioassay method. Compounds 2 — 5 and 9 having the sp 3 atom at C-8 showed an allergy-preventive effect, while compounds 6 , 7 and 8 having a double bond at C-7, C-8 did not.
关键词：Lonicera japonica;allergy-preventive effect;blood flow;chlorogenic acid;iridoid derivative;hen-egg white lysozyme
