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  • 标题:Resveratrol-supplemented holding or re-culture media improves viability of fresh or vitrified-warmed in vitro-derived bovine embryos
  • 本地全文:下载
  • 作者:Arianny Rafaela Neto Silva ; Thaisa Campos Marques ; Elisa Caroline Silva Santos
  • 期刊名称:Research, Society and Development
  • 电子版ISSN:2525-3409
  • 出版年度:2021
  • 卷号:10
  • 期号:14
  • 页码:1-13
  • DOI:10.33448/rsd-v10i14.22097
  • 语种:English
  • 出版社:Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
  • 摘要:The effect of resveratrol supplementation on fresh (E1) or vitrified/warmed (E2) in vitro produced bovine embryos was investigated by evaluating the time-dependent response. After in vitro production, resveratrol (0.5 μM) was added to the incubation media and after two incubation periods with or without resveratrol, blastocysts were re-cultured for 24h. The rates of re-expansion, hatching, total cell number (TCN), apoptotic cells (ACN), reactive oxygen species (ROS) and intracellular glutathione (GSH) content were evaluated. For E1, the re-expansion rate differed at 6 and 10h within and between treatments (Pin vitro produced bovine embryos has a positive and time-dependent effect. The reduction of ROS content, the increase of GSH and the anti-apoptotic ability of resveratrol are responsible for its protective effects, allowing an extension of embryo storage time before transfer to recipients.
  • 关键词:Antioxidant effect;Embryonic development;Holding;In vitro production;Warming.
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