出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：The objective was to evaluate the toxicity of tinctures of mallow 10% (Malva sylvestris) and marigold 10% (Calendula officinalis), through the bioassay with Artemia salina. The nauplii were obtained and transferred to test tubes, which contained mallow and marigold tinctures in different concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/ml), and the control substances (saline water and 0.12% chlorhexidine. The test was performed in triplicate and live nauplii were counted after 24 h. The mean lethal concentration (LC50) was obtained and submitted to Probit analysis using the StatPlus software. Survival curves and LogRank tests were performed for the tinctures compared to controls, using the GraphPad Prism software. The LC50 (μg/ml ± standard deviation) for mallow was 276.08 ± 41.12, and for marigold 268.95 ± 44.09, both tinctures being considered active. There was a statistical difference in the survival curve for both mallow and marigold when compared to saline solution (p<0.0001), indicating greater toxicity of the tinctures. There was also a statistical difference when the tinctures were compared to chlorhexidine (p<0.0001), indicating greater toxicity of chlorhexidine. There was no difference in the survival rate of nauplii, when the mallow and marigold tinctures were analyzed (p = 0.8089). It was concluded that the tinctures of mallow 10% and marigold 10% presented moderate toxicity against Artemia salina.
