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  • 标题:Isolation, molecular characterization and preliminary screening for probiotic properties of lactobacillus fermentum from indigenous dahi
  • 本地全文:下载
  • 作者:Wajiha Farid ; Tariq Masud ; Asma Sohail
  • 期刊名称:E3S Web of Conferences
  • 印刷版ISSN:2267-1242
  • 电子版ISSN:2267-1242
  • 出版年度:2021
  • 卷号:332
  • 页码:1-7
  • DOI:10.1051/e3sconf/202133206004
  • 语种:English
  • 出版社:EDP Sciences
  • 摘要:Indigenous dahi is an analogue to fermented milk product of Pakistan produce by uncharacterized strains of LAB. It hides lots of health beneficial properties and unexplored micro-flora. On microbial examination of indigenous dahi, it was found that L. fermentum was most dominant spp. (22 isolates) of LAB in it. Upon safety assessment eleven showed negative hemolytic and gelatinase activity. All these eleven strains were molecularly characterized through PCR by using universal primers (9F and 1510R). Obtained sequences were submitted to Gene data base of NCBI under accession numbers KX944639-42, KX957930-33 and KY069971-73. Furthermore these strains were screened on the basis of acid and bile tolerance and antimicrobial activity. KX957931, KX957932 and KY069973 were selected for further studies of functional attributes.
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