摘要：SummaryLactic acid bacteria (LAB) influence multiple aspects of host brain function via the production of active metabolites in the gut, which is known as the pre/probiotic action. However, little is known about the biogenic effects of LAB on host brain function. Here, we reported that theLactobacillus plantarumSBT2227 promoted sleep inDrosophila melanogaster. Administration of SBT2227 primarily increased the amount of sleep and decreased sleep latency at the beginning of night-time. The sleep-promoting effects of SBT2227 were independent of the existing gut flora. Furthermore, heat treatment or mechanical crushing of SBT2227 did not suppress the sleep-promoting effects, indicative of biogenic action. Transcriptome analysis and RNAi mini-screening for gut-derived peptide hormones revealed the requirement of neuropeptide F, a homolog of the mammalian neuropeptide Y, for the action of SBT2227. These biogenic effects of SBT2227 on the host sleep provide new insights into the interaction between the brain and gut bacteria.Graphical abstractDisplay OmittedHighlights•Lactobacillus plantarumSBT2227 promotes sleep at the onset of nighttime•Existing intestinal microbes do not affect the SBT2227 sleep effect•Heat-stable intracellular/intramembrane components are candidates for active substances•Neuropeptide Fis required for the sleep-promoting effect of SBT2227Microbiology; Microbiome; Omics
