摘要：SummaryHarmfulMicrocystisblooms (HMBs) and microcystins (MCs) that are produced by Microcystisseriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO5exhibits higher loading capacities for crude MlrA (1,559 mg·g−1) and pure MlrA (1,659 mg·g−1). Moreover, the performances of HGO5@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO5treatment alone, have been studied. These results indicate that HGO5can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.Graphical abstractDisplay OmittedHighlights•Hyperoxic graphene oxide (HGO5) provides inherent strain effects•HGO5exhibits an impressive loading capacity for MlrA•A new assembly mechanism for the HGO5@MlrA composite is proposed•HGO5@MlrA composite shows excellent capability and ecological safetyApplied microbiology; Materials science; Materials chemistry
