摘要：SummaryMicroalgae-based technology is an effective and environmentally friendly method for antibiotics-contaminated wastewater treatment. To assess the tolerance and removal ability ofChlorella sorokinianato ciprofloxacin (CIP), this study comprehensively revealed the responses ofC. sorokinianato CIP exposure and its degradation processes through physiological and transcriptomic analyses. Although the photosynthetic system was inhibited, the growth ofC. sorokinianawas not negatively affected by CIP. Dissolved organic matter was analyzed and indicated that humic-like substances were released to alleviate the stress of CIP. In addition, the maximum removal of CIP was 83.3% under 20 mg L−1CIP exposure. HPLC-MS/MS and RNA-Seq analyses suggested that CIP could be bioaccumulated and biodegraded byC. sorokinianathrough the reactions of hydroxylation, demethylation, ring cleavage, oxidation, dehydrogenation, and decarboxylation with the help of intracellular oxidoreductases, especially cytochrome P450. Collectively, this research shows thatC. sorokinianahave a great potential for removing CIP from wastewater.Graphical abstractDisplay OmittedHighlights•Freshwater microalgaeChlorella sorokinianawas exposed to ciprofloxacin•Microalgae released humic-like substances to alleviate the damage•The maximum removal of ciprofloxacin was 83.3% under 20 mg L−1CIP exposure•Ciprofloxacin could be biodegraded with the help of intracellular oxidoreductasesEnvironmental biotechnology; Applied microbiology; Microorganism
