摘要：SummaryThe morphology of collagen-producing cells and the structure of produced collagen in the dermis have not been well-described. This lack of insights has been a serious obstacle in the evaluation of skin regeneration. We succeeded in visualizing collagen-producing cells and produced collagen using the axolotl skin, which is highly transparent. The visualized dermal collagen had a lattice-like structure. The collagen-producing fibroblasts consistently possessed the lattice-patterned filopodia along with the lattice-patterned collagen network. The dynamics of this lattice-like structure were also verified in the skin regeneration process of axolotls, and it was found that the correct lattice-like structure was not reorganized after simple skin wounding but was reorganized in the presence of nerves. These findings are not only fundamental insights in dermatology but also valuable insights into the mechanism of skin regeneration.Graphical abstractDisplay OmittedHighlights•Dermal collagen synthesized by a single cell was visualized in the axolotl skin•Collagen-synthetic cells were visualized and revealed lattice-patterned filopodia•Collagen pattern was deformed after simple skin wounding•The lattice-patterned collagen was only restorable in the presence of nervesPhysiology; Animal physiology; Bioengineering
