摘要：SummaryAdipose tissue inflammation drives obesity-related cardiometabolic diseases. Enhancing endogenous resolution mechanisms through administration of lipoxin A4, a specialized pro-resolving lipid mediator, was shown to reduce adipose inflammation and subsequently protects against obesity-induced systemic disease in mice. Here, we demonstrate that lipoxins reduce inflammation in 3D-cultured human adipocytes and adipose tissue explants from obese patients. Approximately 50% of patients responded particularly well to lipoxins by reducing inflammatory cytokines and promoting an anti-inflammatory M2 macrophage phenotype. Responding patients were characterized by elevated systemic levels of C-reactive protein, which causes inflammation in cultured human adipocytes. Responders appeared more prone to producing anti-inflammatory oxylipins and displayed elevated prostaglandin D2 levels, which has been interlinked with transcription of lipoxin-generating enzymes. Using explant cultures, this study provides the first proof-of-concept evidence supporting the therapeutic potential of lipoxins in reducing human adipose tissue inflammation. Our data further indicate that lipoxin treatment may require a tailored personalized-medicine approach.Graphical abstractDisplay OmittedHighlights•3D cell culture systems amplify lipoxin-mediated responses in human adipocytes•Lipoxins reduce obesity-related inflammation in human adipose tissue explants•The amplitude of a lipoxin-mediated response correlates with a patient’s plasma CRP level•Lipoxin responders have altered inflammatory and adipose tissue lipid profilesPrecision medicine; Biological sciences; Physiology; Human metabolism; Cell biology
