摘要：SummaryMultiple pesticide residue accumulations increase the probability of chronic metabolic diseases in humans. Thus, we applied multi-omics techniques to reveal how the gut microbiome responded to pesticide exposure. Then, we explored how probioticLactiplantibacillus plantarumP9 (P9) consumption impacted the gut microbiota and immune factors after high pesticide exposure. Multi-omics results indicated frequent exposure to pesticides did not alter the composition of the intestinal microbiota, but it did increase the abundance of Lipopolysaccharide in the gut, which might contribute to chronic inflammation. Supplementation with P9 maintained the homeostasis of the gut microbiota and reduced the abundance of pathogens in the high pesticide-exposed subjects. By detecting metabolites, we observed uridine and 5-oxoproline concentrations increased significantly after P9 consumption. Furthermore, P9 alleviated immune factors disorder and promoted pesticide residue excretion. Our findings provide new insights into the application of probiotics for pesticide detoxification, and suggest probiotics as daily supplements for pesticide exposure prevention.Graphical abstractDisplay OmittedHighlights•High-frequency pesticide exposure induced inflammatory responses to occur•P9 maintained gut microbiota homeostasis in subjects with high pesticide exposure•P9 significantly increased the level of beneficial metabolites in the subjects•P9 reduced inflammatory response and promoted excretion of pesticide residuesBiological sciences; Microbiology; Omics
