Treatment of 1,2-cyclohexanedione with 1,2-diamines, e.g. ethylenediamine and cis -(and trans -)1,2-diaminocyclohexane, caused [4+2] cyclocondensation to give the corresponding dihydropyrazine derivatives (compounds 1—6). They exhibited stronger DNA strand-breakage activity than that of dihydropyrazines, which has already been reported in previous papers.