出版社：Japanese Association of Forensic Science and Technology
摘要：In Japan, possession of germinable cannabis seeds for cultivation purposes is subject to prosecution. Cannabis seeds are marketed after being treated to prevent germination (heated or crushed). Currently, forensic examination of cannabis seeds is conducted by cultivating the seeds after germination tests for several weeks and then performing morphological observation and chemical analysis on the true leaves. In this study, we attempted to construct a rapid and simple method for the identification of cannabis seeds by combining the color reaction using 2,3,5-Triphenyl-2H-tetrazolium Chloride (TTC), a reagent that discriminates between living and dead cells, and DNA testing using a commercially available simple kit.
关键词：Cannabis sativa L.;
Color reaction;
2,3,5-Triphenyl-2H-tetrazolium Chloride;
Germination ability;
Cannabis DNA detection kit
