期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2022
卷号：119
期号：26
DOI：10.1073/pnas.2205850119
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Significance
Bee pollinator pesticide risk assessment is a regulatory requirement for pesticide registration and is largely based on experimental data collected for surrogate species such as the western honeybee. Recently, CYP9Q3, a honeybee cytochrome P450 enzyme, has been shown to efficiently detoxify certain insecticides such as the butenolide flupyradifurone and the neonicotinoid thiacloprid. Here we analyzed genomic data for 75 bee species and demonstrated by the recombinant expression of 26 CYP9Q3 putative functional orthologs that this detoxification principle is an evolutionary conserved mechanism across bee families. Our toxicogenomics approach has the potential to inform pesticide risk assessment for nonmanaged bee species that are not accessible for acute toxicity testing.
The regulatory process for assessing the risks of pesticides to bees relies heavily on the use of the honeybee,
Apis mellifera, as a model for other bee species. However, the validity of using
A. mellifera as a surrogate for other
Apis and non-
Apis bees in pesticide risk assessment has been questioned. Related to this line of research, recent work on
A. mellifera has shown that specific P450 enzymes belonging to the CYP9Q subfamily act as critically important determinants of insecticide sensitivity in this species by efficiently detoxifying certain insecticide chemotypes. However, the extent to which the presence of functional orthologs of these enzymes is conserved across the diversity of bees is unclear. Here we used a phylogenomic approach to identify > 100 putative CYP9Q functional orthologs across 75 bee species encompassing all major bee families. Functional analysis of 26 P450s from 20 representative bee species revealed that P450-mediated detoxification of certain systemic insecticides, including the neonicotinoid thiacloprid and the butenolide flupyradifurone, is conserved across all major bee pollinator families. However, our analyses also reveal that
CYP9Q-related genes are not universal to all bee species, with some Megachilidae species lacking such genes. Thus, our results reveal an evolutionary conserved capacity to metabolize certain insecticides across all major bee families while identifying a small number of bee species where this function may have been lost. Furthermore, they illustrate the potential of a toxicogenomic approach to inform pesticide risk assessment for nonmanaged bee species by predicting the capability of bee pollinator species to break down synthetic insecticides.
