期刊名称：Scientific Annals of the Danube Delta Institute
印刷版ISSN：1842-614X
电子版ISSN：2247-9902
出版年度：2022
卷号：27
DOI：10.7427/DDI.27.04
语种：English
出版社：Institutul National de Cercetare-Dezvoltare Delta Dunarii
摘要：Biodiversity assessment is crucial for evaluating the ecological status of freshwater ecosystems. Macroinvertebrates are one of the ecological indicators used for water quality assessment. In many cases, morphological identification of macroinvertebrates poses problems when different life stages need to be identified to the species level. A molecular approach is needed for assigning individuals to a species. In this order, 12 samples of two taxonomic groups, order Diptera (Family Chironomidae) and order Coleoptera collected in the Danube Delta were analyzed using a short sequence of mtDNA COI barcode. Ten samples were successfully identified to species level based on 97-100% similarity with DNA sequences from GenBank and BOLD databases. One sample could not be assigned to any species and for one sample no DNA correspondent was found in both databases. Overall, this preliminary study underlines the importance of DNA barcoding for identification of macroinvertebrates species and brings new light on the biodiversity assessment of Danube Delta aquatic macroinvertebrates.
