期刊名称：International Journal of Early Childhood Special Education
电子版ISSN：1308-5581
出版年度：2022
卷号：14
期号：3
页码：3317-3327
DOI：10.9756/INT-JECSE/V14I3.412
语种：English
出版社：International Journal of Early Childhood Special Education
摘要：Ficus benghalensis, a large evergreen tree, belongs to the family Moraceae. It is commonly known as “Indian Banyan Tree”. Antioxidants protect the body against oxidative stress by neutralizing free radicals and reactive oxygen species (ROS) for example, superoxide radicals, hydroxyl radicals, hydrogen peroxide radicals. This oxidative damage produces a lot of chronic human diseases like diabetes mellitus, cancer, atherosclerosis, arthritis, and neurodegenerative diseases. Nanoparticles (NPs) have shown notable advances owing to a wide range of applications in biomedicine, sensors, antimicrobial agents, catalysts, electronics, optical fibers, agricultural, biolabeling, and other areas. The aim of this study is to synthesize the Ficus benghalensis mediated copper nanoparticle and to see its antioxidant activity. The plant extract was prepared using the bark of Ficus benghalensis and mixed with copper nanoparticles. 0.507 g of anhydrous copper sulphate was added to 70 mL of distilled water and 30 mL of plant extract. It was kept in an orbital shaker for uniform dispersion. It was centrifuged and pellets were collected. The pellets obtained were used for antioxidant activity, DPPH assay. For DPPH assay, diverse concentrations of Ficus benghalensis bark extract interceded copper nanoparticles and 1 ml of 0.1 mM DPPH in methanol. It was incubated for 30 minutes. The reduction of DPPH free radicals was assessed dependent on the absorbance at 517 nm. The results were collected and statistically analyzed. The percentage of inhibition of DPPH free radicals was calculated. The values were 64.2% at 10 µL concentration, 63.1% at 20 µL, 55.5% at 30 µL, 46.2% at 40 µL (85.2%) and 46.7% at 50 µL. As the concentration increases the antioxidant activity of the nanoparticles increases. Percentage of Inhibition was highest at 50µL. The values for antioxidant properties of nanoparticles were found to be higher than the standard values at all concentrations except at 50 µL. This study was done to find a way for a greener approach of synthesizing the copper nanoparticles, thereby giving pharmacological evidence against the antioxidant activity. The copper nanoparticle synthesized using Ficus benghalensis has potent free radical scavenging activity, hence has antioxidant property and has a wide array of medical and dental applications.
