期刊名称：International Journal of Early Childhood Special Education
电子版ISSN：1308-5581
出版年度：2022
卷号：14
期号：5
页码：212-219
DOI：10.9756/INTJECSE/V14I5.19
语种：English
出版社：International Journal of Early Childhood Special Education
摘要：Lutein is an antioxidant which is a fat-soluble xanthophyll and could be found in a wide range of natural sources. A variety of separation and analytical methods have been developed for the determination of lutein, including solvent extraction, supercritical fluid extraction, and high performance liquid chromatography. There are many sources for lutein extraction but the search for a more economical source still exists. Moreover, the cost of production of lutein depends on the availability of the source and also the quantity of lutein produced from each source. The cost of lutein is very high and it also depends on the availability of the source and the cost incurred in its production. The objective of the present study is to extract and estimate the lutein present in a variety of flowers available in the vicinity of the institute in a view to identity a cheaper source and to reduce the overall production cost. The plants selected for the study were Senna bicapsularis, Tecoma stans, Caesalpinia pulcherrima (red), Caesalpinia pulcherrima (yellow) and Tagetus erecta. Solvent extraction was carried out using petroleum ether, benzene, diethyl ether and chloroform. Among the solvents used, chloroform was found to be the best solvent. The extracted lutein was estimated using UV-Vis spectrophotometer at 445 nm. The lutein content was found to be the highest in Senna bicapsularis when chloroform was used as the solvent (1217.2 μg / g) and the lowest is Tecoma stans when benzene was used as the solvent (30.74 μg / g). T. erectus and Tecoma stans preformed well with respect to lutien extraction when diethyl ether was used as solvent. When chloroform was used, S. bicapsularis and T. stans showed good result. Similarly, in benzene, T. erectus and S. bicapsularis showed good amounts of lutein. Finally in petroleum ether all plants showed significant amounts of lutein.
