摘要：The interplay between microorganisms generally plays a vital role in food fermentation. In this study, the mutual influence of
Saccharomyces cerevisiae and
Fructilactobacillus sanfranciscensis, the two predominant microbes in the sourdough ecosystem, were investigated in situ during fermentation. Doughs fermented with
S. cerevisiae,
F. sanfranciscensis, or their combination were compared regarding acid production, microbial density, and volatiles. Furthermore, in situ gene expressions were investigated using RNA-sequencing. The results showed that the presence of
S. cerevisiae had no visible influence on
F. sanfranciscensis, whereas
F. sanfranciscensis facilitated the growth of
S. cerevisiae but affected its volatile production since metabolites such as 3-methyl-1-butanol decreased. The RNA-sequencing demonstrated that
S. cerevisiae significantly changed the gene transcripts implicated in amino acid metabolism in
F. sanfranciscensis and may stimulate its growth suggested by the enrichment of the KEGG pathway of peptidoglycan biosynthesis.
