标题：Effect of aromatic oils on the expression of some virulence-associated and antimicrobial resistance genes of Escherichia coli isolated from broilers
期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2022
卷号：9
期号：2
页码：191-202
DOI：10.5455/javar.2022.i584
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objectives:
This study aimed to prove the effects of
Escherichia coli isolates isolated from diseased broilers to form biofilms, describe their antimicrobial sensetivity, and determine the effect of allicin and cinnamon essential oils on the expression of some genes (
fimH,
int1, and
luxS) through quantitative polymerase chain reaction (q-PCR).
Materials and Methods:
140 samples were obtained from diseased broilers in Beni-Suef Governorate, Egypt. These samples were examined by conventional bacteriology methods to detect the causative agent. The antimicrobial susceptibility of the isolated bacteria was assessed using the disc diffusion method, The ability of yeast extract-casamino acids Congo Red Agar to generate phenotypic biofilms was next tested. The presence of resistance and virulence genes in some multidrug resistant isolates was genotypically investigated. The antibacterial effects of allicin and cinnamon oil were evaluated against the growth of multidrug-resistant
E. coli. Finally, q-PCR was utilized to assess changes in some genes’ expression.
Results:
Escherichia coli was isolated from 61 samples (43.6%). An antimicrobial susceptibility test revealed that multidrug-resistance (MDR) (could resist more than three antimicrobial classes)
E. coli prevalence was 100%. 40.8% of isolates phenotypically produce biofilms. The detection of resistance and virulence genes by PCR showed that all tested isolates carry
aadB,
fimH,
int1,
qnrS, and
luxS genes, while only 40% harbor
iss genes. q-PCR showed that after treatment with allicin and cinnamon oils, gene expression went down.
Conclusion:
This investigation highlights that
E. coli showed resistance against most of the tested antimicrobials; all isolates were MDR. The study showed wide dissemination of virulence and resistance genes among
E. coli. Allicin and cinnamon oils have antimicrobial activities and could be used as alternatives to synthetic antimicrobial agents.
