期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2022
卷号：9
期号：2
页码：267-274
DOI：10.5455/javar.2022.i592
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objective:
The effect of
Artemisia herba-alba methanolic extract monotherapy and combination therapies on the
in vitro growth of several
Babesiaand Theileria parasites
in vitroand mice was investigated in this study.
Materials and Methods:
Fluorescence assay using SYBR Green I stain was used to evaluate the antibabesial efficacy inhibitory of
A. herba-alba either
in vitro or
in vivo. Hematological parameters in the treated mice were analyzed using a Celltac MEK-6450 computerized hematology analyzer.
Results:
Artemisia herba-alba reduced the growth of
Babesia bovis,
Babesia bigemina,
Babesia divergens,
Theileria equi, and
Babesia caballi in vitro in a dose-dependent manner. The
in vitro inhibitory impact of
A. herba-alba on
B. divergens and
B. caballi cultures was amplified when combined with either diminazene aceturate (DA). In
B. microti-infected mice, a combination therapy consisting of
A. herba-alba and a low DA dose inhibited
B. microti growth significantly (
p < 0.05) better than treatment with 25 mg kg
−1 DA.
Conclusions:
These data show that
A. herba-alba, when paired with a modest DA dose, could be a promising medicinal plant for babesiosis treatment.
