Air quality in demolition practices has seldom been evaluated in Iran. Accordingly, we evaluated asbestos exposure among Tehran construction workers during the demolition of old houses. To identify possible sources of asbestos exposure, including thermal insulations, chimney pipes and cement sheets, were all sampled. This study also were taken the personal air samples to evaluate any asbestos exposure during the demolition. The asbestos fibers found in the samples were analyzed by phase-contrast optical microscopy (PCM), scanning electron microscopy (SEM) equipped with an energy dispersive X-ray analysis, and polarized light microscopy (PLM) methods. Personal monitoring of asbestos fiber levels indicated a range from 0.01 to 0.15 PCM f/ml (0.02–0.42 SEM f/ml). The geometric mean concentrations were 0.07 PCM f/ml (0.20 SEM f/ml), which is considerably higher than the threshold limit value (TLV) proposed by American Conference of Governmental Industrial Hygienist (ACGIH), which is 0.1 f/ml. The analysis showed a presence in the bulk samples only chrysotile asbestos and an absence of the other type asbestos. Therefore, it might be expected that workers who worked in the demolition of old houses will suffer from negative effects of exposing to the asbestos fibers.