摘要：SummaryAlthough emerging evidence reveals that vaping alters the function of the central nervous system, the effects of maternal vaping on offspring brain development remain elusive. Using a well-establishedin uteroexposure model, we performed single-nucleus ATAC-seq (snATAC-seq) and RNA sequencing (snRNA-seq) on prenatally e-cigarette-exposed rat brains. We found that maternal vaping distorted neuronal lineage differentiation in the neonatal brain by promoting excitatory neurons and inhibiting lateral ganglionic eminence-derived inhibitory neuronal differentiation. Moreover, maternal vaping disrupted calcium homeostasis, induced microglia cell death, and elevated susceptibility to cerebral ischemic injury in the developing brain of offspring. Our results suggest that the aberrant calcium signaling, diminished microglial population, and impaired microglia-neuron interaction may all contribute to the underlying mechanisms by which prenatal e-cigarette exposure impairs neonatal rat brain development. Our findings raise the concern that maternal vaping may cause adverse long-term brain damage to the offspring.Graphical abstractDisplay OmittedHighlights•Prenatal e-cigarette exposure adversely affects rat brain development•Prenatal e-cigarette smoking disrupts brain excitatory/inhibitory neuron balance•Prenatal e-cigarette smoking disrupts Ca2+homeostasis, induces microglia cell death•Prenatal e-cigarette exposure increases susceptibility to brain ischemic injuryBiological sciences; Neuroscience; Developmental neuroscience; Systems neuroscience; Omics; Transcriptomics
