标题：The Biophysical Examination Employing Multi Spectroscopic And Molecular Docking Techniques To Investigate The Interaction Of Bioactive Quercetin And Beta Carotene With Lysozyme
摘要：The binding connection between the small anticancer drug Quercetin/Beta-Carotene and lysozyme has been investigated using multi-spectroscopic and computational methods (Lys). This research seeks to investigate the binding mechanism of Quercetin and Beta-Carotene to lysozyme using multi-spectroscopic and molecular docking techniques. The creation of hamiltonian complexes involving lysozyme and Quercetin and Beta-Carotene has been demonstrated using UV–Vis and steady-state fluorescence spectroscopy. Stern-Volmer simulations were used to assess the relative change in fluorescent intensity as a function of medication concentrations. According to the outcomes of emission and focused fluorescence experiments, the quenching of lysozyme emmisions with Quercetin and Beta-Carotene is begun by a static quenching process. In addition, research using absorbency; fixed frequency synchronous fluorescence, fluorescence, and Fourier-transform infrared (FT-IR) demonstrated that Quercetin and Beta-Carotene produced structural alterations in the lysozyme protein sequence. The results were then confirmed by molecular docking data, which revealed the specific mode of contact. According to molecular docking study, the potential binding site of click is at the Tyr and Trp residues of lysozyme, and the major factors responsible for the complexation of Quercetin and Beta-Carotene with lysozyme include the hydroxyl group and hydrophobic contact.
