期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2005
卷号：37
期号：2
页码：45-53
DOI：10.3164/jcbn.37.45
出版社：The Society for Free Radical Research Japan
摘要：Thioredoxin is a vital component of the thioredoxin system, maintaining the cellular redox environment and regulating various cellular functions. Studies using thioredoxin transgenic mice showed that thioredoxin is protective against diseases associated with oxidative stress. While thioredoxin expression is ubiquitous and constitutive, we observe the induction in some tissues under stress such as ultraviolet irradiation and reperfusion injury. Nerve growth factor (NGF)-, or heme-induced induction of the thioredoxin gene is regulated through cyclic AMP responsive element (CRE) or the antioxidant responsive element (ARE), respectively. Based on these previous studies, we have performed a screening of extracts from vegetables to identify novel thioredoxin inducers. We showed that sulforaphane and extracts from several kinds of vegetables induce marked activation of the thioredoxin gene through ARE and increase the thioredoxin protein level in K562 erythroleukemia cells and retinal pigment epithelial (RPE) cells. Pretreatment with sulforaphane or the extracts from sprouts of red cabbage reduced cellular damage induced by hydrogen peroxide. We also report the effect of sulforaphane on the photooxidative damage of RPE cells. Thioredoxin inducers and thioredoxin have a cytoprotective role against oxidative stress in gastric mucosal cells. Reduction of disulfide bonds in food allergens was reported to facilitate the cleavage by digestive enzymes. These results provide a basis for developing thioredoxin-inducing and containing functional foods and further investigating thioredoxin inducers beneficial for protection against oxidative stress.
关键词：thioredoxin;sulforaphane;oxidative stress;antioxidant responsive element (ARE)
