摘要：Resveratrol (RES) has a wide range of biological and pharmacological activities with various health benefits for humans as a food additive. In animal production, RES has been considered a potential functional feed additive for producing high-quality pork. Long noncoding RNAs (lncRNAs) have emerged as essential regulators of fat metabolism, and phytochemicals can regulate fat metabolism through lncRNA. However, it is unclear whether RES can improve back-fat thickness by regulating lncRNA. In this study, we identified a novel lncRNA, which was named a long intergenic non-protein coding RNA, a regulator of fat metabolism (
LincRNA-ROFM), from our previous lncRNA sequencing data.
LincRNA-ROFM can inhibit adipocyte proliferation and differentiation. In-depth analyses showed that
LincRNA-ROFM acts as a molecular sponge for
miR-133b, and adiponectin (AdipoQ) is a direct target of
miR-133b in porcine preadipocytes. In addition, the expression of
LincRNA-ROFM was positively correlated with AdipoQ. RES can promote the expression of
LincRNA-ROFM by
PPARα and
C/EBPα. Altogether, our research showed that
LincRNA-ROFM acts as a ceRNA to sequester
miR-133b and is upregulated by RES, leading to heightened AdipoQ expression, and thus decreased adipocyte proliferation and differentiation, which reduces back-fat thickness of pigs. Taken together, the
RES/LincRNA-ROFM/miR-133b/AdipoQ regulatory network preliminarily explains the mechanism of action of RES in inhibiting fat deposition, which provides new insight into the downstream mechanism of RES inhibition of fat deposits by regulating the lncRNA.
