摘要：Distribution of immunocompetent cells in lymphoid tissues of healthy nulliparous horses were revealed by the double-staining indirect immunofluorescence antibody technique with monoclonal antibodies. In the thymus, immature T cells (CD4+ 8+ T cells) aggregated in the cortex, while a few mature T cells (CD4+ 8- or CD4- 8+ T cells) were scattered in the medulla. MHC class II+ cells aggregated in the cortico medullary region were considered to be macrophages and dendritic cells, while the MHC class II+ cells in the cortex were thought to be macrophages, dendritic cells or cortical epithelial cells. The existence of T cell-dependent areas in the spleen, lymph nodes and ileum was confirmed by the presence of CD5+ T cells. The number of helper T cells (CD4+ 5+ T cells) was slightly greater than that of killer T cells (CD5+ 8+ T cells) in these areas. Within the follicles of the spleen, lymph nodes and ileum, aggregated MHC class II+ cells were considered to be B cells and dendritic cells. Almost all the IgM+ MHC class II+ B cells aggregated within the center of the follicles, whereas IgM- MHC class II+ B cells were found in the marginal zone of the follicles. These findings suggest that activated B cells are contained in the center of the follicle and inactivated B cells in the marginal zone of the follicle and that the follicles are adjoined to T cell-dependent areas.
