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  • 标题:Identification of the Gene Encoding VP7 of Serotype G3 Equine Rotavirus
  • 本地全文:下载
  • 作者:Kenji TSUBOKURA ; Hiroyuki HORIUCHI ; Seiji SATO
  • 期刊名称:Journal of Equine Science
  • 印刷版ISSN:1340-3516
  • 电子版ISSN:1347-7501
  • 出版年度:1996
  • 卷号:7
  • 期号:1
  • 页码:17-20
  • DOI:10.1294/jes.7.17
  • 出版社:Japanese Society of Equine Science
  • 摘要:The sequence of the VP7-encoded gene of a serotype G3 equine rotavirus strain HO-5 isolated in Japan was determined. The VP7 was amplified by a reverse transcription-polymerase chain reaction from the segment 8 RNA of the HO-5 strain by using a synthetic primer pair for the rotavirus VP7 gene. The gene segment 8 of the HO-5 strain was also sequenced and the deduced amino acid sequence was determined. The results indicate that the VP7 of the HO-5 strain is encoded by the gene segment 8 and the deduced amino acid sequence of the HO-5 VP7 has an overall high sequence homology more than 95% to those of two known equine rotavirus strains.
  • 关键词:equine;rotavirus;sequence;VP7
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