标题：Identification of the Major Epitope in the GL Protein of Equine Arteritis Virus (EAV) Recognized by Antibody in EAV-infected Horses Using Synthetic Peptides
摘要：A panel of overlapping peptides covering the ectodomain of the GL protein of equine arteritis virus (EAV) was synthesized to identify the major epitope recognized by the EAV-seropositive horse sera by using enzyme-linked immunosorbent assay (ELISA). Three consecutive peptides, G15 (amino acid residues 75-90), G16 (79-94) and G17 (83-98), strongly reacted with 3 experimentally EAV-infected horse sera. G16 peptide was highly antigenic with all 8 EAV-infected horse sera tested. The ELISA absorbance values and the virus neutralization titers of antibodies in sera periodically collected from EAV-infected horses showed similar patterns. These results indicated that the major linear epitope of the EAV GL protein which was recognized with EAV-infected horse sera was mapped to residues from 83 to 90.
