摘要:Rabbits have been widely used for studying ocular physiology and pathology due to their relatively large eye size and similar structures with human eyes . Various rabbit ocular disease models, such as dry eye, age-related macular degeneration, and glaucoma, have been established . Despite the growing application of proteomics in vision research using rabbit ocular models, there is no spectral assay library for rabbit eye proteome publicly available . Here, we generated spectral assay libraries for rabbit eye compartments, including conjunctiva, cornea, iris, retina, sclera, vitreous humor, and tears using fractionated samples and ion mobility separation enabling deep proteome coverage . The rabbit eye spectral assay library includes 9,830 protein groups and 113,593 peptides . We present the data as a freely available community resource for proteomic studies in the vision feld . Instrument data and spectral libraries are available via ProteomeXchange with identifer PXD031194 .