摘要：We present a dataset of microelectrode array (MEa) recordings from human pluripotent stem cell (hPSC)-derived and rat embryonic cortical neurons during their in vitro maturation. the data were prepared to assess extracellularly recorded spontaneous activity and to compare the functional development of these neuronal networks. In addition to recordings of spontaneous activity, we provide pharmacological responses of hPSC-derived and rat cortical cultures at their mature stage. together with the recorded electrode raw data, we share the analysis code to form a comprehensive dataset including spike times, spike waveforms, burst activity and network synchronization metrics calculated with two diferent connectivity estimators . Moreover, we provide the analysis code that produced the key scientifc fndings published previously with this dataset . This large dataset enables investigation of the functional aspects of maturing cortical neuronal networks and provides substantial parameters to assess the diferences and similarities between hPSC-derived and rat cortical networks in vitro . This publicly available dataset will be benefcial, especially for experimental and computational neuroscientists.
