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  • 标题:A chromosome-level genome assembly of the jade perch (Scortum barcoo)
  • 本地全文:下载
  • 作者:Yishan Lu ; Ruihan Li ; Liqun Xia
  • 期刊名称:Scientific Data
  • 电子版ISSN:2052-4463
  • 出版年度:2022
  • 卷号:9
  • 期号:1
  • 页码:1-10
  • DOI:10.1038/s41597-022-01523-y
  • 语种:English
  • 出版社:Nature Publishing Group
  • 摘要:Endemic to Australia, jade perch (Scortum barcoo) is a highly proftable freshwater bass species . It has extraordinarily high levels of omega-3 polyunsaturated fatty acids (PUFAs), which detailed genes involved in are largely unclear. Meanwhile, there were four chromosome-level bass species have been previous sequenced, while the bass ancestor genome karyotypes have not been estimated . Therefore, we sequenced, assembled and annotated a genome ofjade perch to characterize the detailed genes for biosynthesis of omega-3 PUFAs and to deduce the bass ancestor genome karyotypes . We constructed a chromosome-level genome assembly with 24 pairs of chromosomes, 657.7 Mb in total length, and the contig and the scafold N50 of 4.8 Mb and 28.6 Mb respectively. We also identifed repetitive elements (accounting for 19 .7% of the genome assembly) and predicted 26,905 protein-coding genes . Meanwhile, we performed genome-wide localization and characterization of several important genes encoding some key enzymes in the biosynthesis pathway of PUFAs . These genes may contribute to the high concentration of omega-3 in jade perch . Moreover, we conducted a series of comparative genomic analyses among four representative bass species at a chromosome level, resulting in a series of sequences of a deductive bass ancestor genome .
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