首页    期刊浏览 2024年11月27日 星期三
登录注册

文章基本信息

  • 标题:Optimum Conditions for Derivatization of Glutathione, Cysteine and Cysteinylglycine in Human Plasma with Ammonium 7-Fluorobenzo-2-Oxa-1,3-Diazole-4-Sulfonate for Accurate Quantitation by High-Performance Liquid Chromatography
  • 作者:Yoshikazu Yoshida ; Yasuharu Ohiwa ; Masao Shimamura
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:2003
  • 卷号:49
  • 期号:6
  • 页码:527-530
  • DOI:10.1248/jhs.49.527
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:For the accurate quantitation of glutathione (GSH), cysteine (Cys) and cysteinylglycine (CysGly) in human plasma by detection of fluorescence after derivatization with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F), a thiol-specific fluorogenic agent and high-performance liquid chromatography (HPLC) , we attempted to identify optimum conditions for the derivatization and treatment of samples. The extent of derivatization with SBD-F of endogenous thiol compounds in human plasma was affected by several factors, such as the concentration of SBD-F and the reaction time. Acidification and deproteinization of plasma immediately after the separation of plasma from whole blood were essential and prevented the determination of inappropriately low values. The method developed in the present study allowed recovery that was closed to 100% for each compound. The mean values determined for 61 healthy individuals were as follows: Cys, 50.1 μM; CysGly, 12.2 μM; and GSH, 5.3 μM.
  • 关键词:glutathione;cysteine;cysteinylglycine;human plasma;HPLC
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有