摘要：To isolate the genes regulated by peroxisome proliferator-activated receptor γ (PPAR γ ), we first developed a stable transformant expressing PPAR γ . Using cytosol and nuclear fractions prepared from this cell line, we performed two-dimensional electrophoresis. A comparison of the electrophoretic patterns with those from control cells revealed many spots to be up-regulated or down-regulated proteins. Some spots were subjected to mass spectrometric analyses, and triosephosphate isomerase and galectin-3 were identified as proteins down-regulated by PPAR γ . Moreover, Northern blot analyses revealed that the expression level of both genes decreased in PPAR γ -expressing cells. These results strongly suggest that triosephosphate isomerase and galectin-3 are target genes whose expression is mediated by PPAR γ .
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