摘要：The effect of β -cryptoxanthin on bone components in the femoral tissues of aged rats was investigated. β -Cryptoxanthin was isolated from Satsuma mandarin ( Citrus unshiu MARC.). β -Cryptoxanthin (5, 10, or 20 μ g/100 g body weight) was orally administered once daily for 7 days to aged (50 week old) female rats. The administration of β -cryptoxanthin (10 or 20 μ g/100 g body weight) caused a significant increase in calcium content, alkaline phosphatase activity and deoxyribonucleic acid (DNA) content in the femoral-diaphyseal and -metaphyseal tissues. Femoral-diaphyseal calcium content and alkaline phosphatase activity were significantly increased by the dose of 5 μ g/100 g body weight. A significant increase in metaphyseal alkaline phosphatase activity was also seen with the dose of 5 μ g β -cryptoxanthin/100 g body weight. Moreover, bone tissues were cultured for 48 hr in serum-free Dulbecco's modified Eagle's medium containing either vehicle or β -cryptoxanthin (10-7 or 10-6 M). The presence of β -cryptoxanthin (10-7 or 10-6 M) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and -metaphyseal tissues obtained from aged (50 week old) female rats. These increases were completely abolished in the presence of cycloheximide (10-6 M), an inhibitor of protein synthesis. This study demonstrated that β -cryptoxanthin has an anabolic effect on bone components in aged female rats in vivo and in vitro .
关键词：β-cryptoxanthin;bone formation;osteoporosis;aged rat femur
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