摘要：TFIIS is a general transcription elongation factor that interacts with Elongin (SIII), a product of the tumor suppressor gene for the human hereditary disorder von Hippel-Lindau (VHL) disease. Here, we have studied the promoter region of the Xenopus general type TFIIS ( XGTFIIS ) gene using a luciferase assay. The previously cloned region 1600-bp upstream of the XGTFIIS gene was divided into 12 sequential deletion fragments, and each fragment was inserted into the pGL3-Basic vector, which was used as a reporter for a luciferase assay in the Xenopus cultured cell-line, XTC. Except for the proximal promoter region close to the transcription start sites, no positively or negatively regulating element was found for TFIIS gene expression within the 1600-bp upstream region of the XGTFIIS gene. Of the two previously identified transcription start sites, the one that is distal to the ATG codon was preferred over the proximal one.
关键词：von Hippel-Lindau disease;Xenopus laevis;general type TFIIS;genomic cloning;transcription start site;luciferase assay
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