首页    期刊浏览 2024年11月26日 星期二
登录注册

文章基本信息

  • 标题:Isolation and Characterization of a 1,3-Dichloro-2-Propanol-Degrading Bacterium
  • 作者:Risa Yonetani ; Hisayoshi Ikatsu ; Chizuko Miyake-Nakayama
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:2004
  • 卷号:50
  • 期号:6
  • 页码:605-612
  • DOI:10.1248/jhs.50.605
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Arthrobacter sp. strain PY1, a bacterium having the ability to degrade 1,3-dichlotopropanol (1,3-DCP), was isolated from a soil sample of a chemical plant. Strain PY1 degraded 1000 mg/l (7.75 mM) of 1,3-DCP completely within 7 days, and the ability was elevated by acclimatization up to 4000 mg/l/week. Addition of nutrients such as peptone, glucose or glycerol showed no or slight effect on the degrading activity. These results suggest that strain PY1 is a useful organism in a biological control system for 1,3-DCP pollution. The ability to degrade 1,3-DCP was induced by addition of 1,3-DCP to the culture of strain PY1. A 1,3-DCP-degrading enzyme (Deh-PY1) was purified from the cytoplasmic fraction of strain PY1 by fractionation with ammonium sulfate, hydrophobic chromatography and anion exchange chromatography. Purified Deh-PY1 is a tetramer of a homogeneous subunit having a molecular weight of 20 kDa. Analysis of the N-terminal amino acid sequence of Deh-PY1 showed that the 31 residues were quite similar to those of known 1,3-DCP-dehalogenases of other organisms, Arthrobacter sp. strain AD2 and Corynebacterium sp. strain N-1074, although some differences in composition or enzymatic characteristics were observed. The Km value and Vmax of Deh-PY1 were 2.67 mM and 7.81 μ mol/min/mg, respectively, and the optimum reaction temperature and pH were 40-50°C and 9.5-10.5.
  • 关键词:1,3-dichloro-2-propanol;biodegradation;Arthrobacter sp.;dehalogenase
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有